Fabian U. Zwettler, Sebastian Reinhard, Markus Sauer
Methods in Cell Biology, Vol. 161, p. 317--340 · 2021
This chapter provides a step-by-step protocol how to prepare expansion microcoscopy (ExM) treated biological samples for imaging with single-molecule localization microscopy (SMLM). For this purpose, the protocol describes the stabilization of expanded hydrogels that enables addition of photoswitching buffer without shrinkage of the sample. In addition, a guide for automated image analysis and expansion factor determination of expanded fiber-like structures is provided at the end of the chapter.
@article{zwettler_ex-dstorm_2021,
title = {Ex-dSTORM and automated quantitative image analysis of expanded filamentous structures},
volume = {161},
issn = {0091-679X},
doi = {10.1016/bs.mcb.2020.05.004},
abstract = {This chapter provides a step-by-step protocol how to prepare expansion microcoscopy (ExM) treated biological samples for imaging with single-molecule localization microscopy (SMLM). For this purpose, the protocol describes the stabilization of expanded hydrogels that enables addition of photoswitching buffer without shrinkage of the sample. In addition, a guide for automated image analysis and expansion factor determination of expanded fiber-like structures is provided at the end of the chapter.},
language = {eng},
journal = {Methods in Cell Biology},
author = {Zwettler, Fabian U. and Reinhard, Sebastian and Sauer, Markus},
year = {2021},
pmid = {33478695},
keywords = {Super-resolution microscopy, dSTORM, Expansion microscopy, Image Processing, Computer-Assisted, LineProfiler, Single Molecule Imaging},
pages = {317--340},
}